Technical Analysis of cDNA Microarrays

Technical Analysis of cDNA Microarrays

BACKGROUND There is extensive variation in gene expression among individuals within and between populations. Accurate measures of the variation in mRNA expression using microarrays can be confounded by technical variation, which includes variation in RNA isolation procedures, day of hybridization and methods used to amplify and dye label RNA for hybridization. METHODOLOGY/PRINCIPAL FINDINGS I...

Analysis of repeatability in spotted cDNA microarrays.

We report a strategy for analysis of data quality in cDNA microarrays based on the repeatability of repeatedly spotted clones. We describe how repeatability can be used to control data quality by developing adaptive filtering criteria for microarray data containing clones spotted in multiple spots. We have applied the method on five publicly available cDNA microarray data sets and one previousl...

Noise factor analysis for cDNA microarrays.

A microarray-image model is used that takes into account many factors, including spot morphology, signal strength, background fluorescent noise, and shape and surface degradation. The model yields synthetic images whose appearance and quality reflect that of real microarray images. The model is used to link noise factors to the fidelity of signal extraction with respect to a standard image-extr...

Fabrication of cDNA microarrays.

Technical Performance of 24sure Microarrays

The ground-breaking 24sure technology delivers aneuploidy screening of 24 chromosomes, within a 12 hour period, starting from single cells. 24sure technology uses a reliable and proven method for amplification of genomic DNA. Amplified samples are then co-hybridized to 24sure or 24sure+ slides—bacterial artificial chromosome (BAC) microarray platforms designed to enable robust and reliable call...